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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Clin Cancer Res. 2012 Aug 2;18(19):5412–5426. doi: 10.1158/1078-0432.CCR-12-1780

Fig. 4. Galanin/GalR1 silencing induces caspase 8-dependent apoptotic cell death.

Fig. 4

Annexin V/PI flow cytometric analysis was used to measure apoptosis levels. (A) HCT116 cells were transfected with 5nM siGALR1 ± 10μM zVAD for 24h prior to a 24h co-treatment with IC30(48h) doses of either 5-FU or oxaliplatin. (B) Caspase 3/7 and 8 activity levels in HCT116 cells following 24h GalR1 silencing (5nM siRNA) prior to a 24h co-treatment with IC30(48h) doses of either 5-FU or oxaliplatin. (C) HCT116 cells following 48h GalR1/galanin (5nM siRNA) and Caspase 8 (10nM siRNA) co-silencing. (C, inset) Western blotting was used to measure expression of PARP, caspase 8 and FLIPL following 24h GalR1 and Caspase 8 co-silencing. (D) HCT116 parental cells and the FLIPL overexpressing cell line, FL17, following 24h GalR1/galanin silencing (5nM siRNA). Results are mean values ± SEM of triplicate measurements. *P<0.05; **P<0.01; ***P<0.001. SC=siRNA control.