Figure 3. Screening rare variants.

(A) Compiled ciliation results for the candidates listed in the abscissa, carried out and indicated in the figure as described in Fig. 2 B. (B) Endogenous expression and candidate gene knockdown assessed by qRT-PCR analysis. All of the siRNA duplexes that produced a ciliation defect were tested for knockdown (blue bars). Green bars indicate duplexes that increased % ciliation, and these were also tested by qRT-PCR. Correlative qRT-PCR analysis was also carried out on all ‘splits’ to determine if the duplex not producing a ciliation defect also failed to knock down expression. Those splits for which this was true (black bars with duplex identity indicated in red) were carried into the second round (the same strategy as described in Fig. 2). (C) Summary list of hits characterized by decreased % ciliation indicated in blue, and hits characterized by increased % ciliation indicated in green.