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. 2012 Oct 3;7(10):e46653. doi: 10.1371/journal.pone.0046653

Figure 4. GFP, GFP-perlucin and perlucin variants achieved by protein expression and purification.

Figure 4

The obtained GFP-perlucin derivatives as identified by MALDI-TOF/TOF (MS+MS/MS), either intact or after trypsin digestion, are His-tagged GFP (A), and truncated GFP-perlucins lacking 141 or 145 (B,C), or 15 C-terminal amino acids (D). Mass fragments of tryptic peptides extracted after SDS-PAGE identified the 46.8 kDa protein as the full length His-tagged GFP-perlucin including the two C-terminal repeat motifs (E). His-tagged perlucin without GFP domain (F) was not analyzed.