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. 2012 Oct 3;7(10):e46653. doi: 10.1371/journal.pone.0046653

Figure 5. Native PAGE of GFP (28.9.

Figure 5

kDa), GFP-perlucin (46.8 kDa), and SDS-PAGE analysis of extracted GFP-perlucin derivatives. Purified GFP yielded one distinct band (A). Affinity-purified GFP-perlucin (B) migrates in 3 separate fluorescent bands (2–4) in native PAGE and another fluorescent species remains in the well (1). The latter contains the full-length GFP-perlucin (46.8 kDa) as demonstrated by denaturing SDS-PAGE under reducing conditions and silver staining (bottom right, amino acid sequence of perlucin (grey) with affinity tag (red) and GFP (green)). Bands 2–4 were identified as truncated versions of the recombinant protein (29.8–30.3 kDa) by MALDI-TOF/TOF (Figure 3). Molecular weight marker (M).