Table 1. MALDI-TOF/TOF (MS+MS/MS) identification of GFP-perlucin (46.8 kDa).
| Sequence | Calculated mass | Expected mass |
| DEDSFIR | 881.3999 | 881.4128 |
| WLWREGQR | 974.4843 | 974.5026 |
| SSFAEAAGYCR | 1161.4993 | 1161.5265 |
| DSLHANLQQR | 1181.6022 | 1181.6262 |
| SCYWFSTIK | 1205.566 | 1205.5936 |
| YLESHLAIISNK | 1387.7579 | 1387.7778 |
| IPYTNSLHANLQQR | 1654.866 | 1654.8976 |
| ERIPYTNSLHANLQQR | 1940.0096 | 1940.0448 |
| LGEAFNYWLGASDLNIEGR | 2125.0349 | 2125.0723 |
| YLESHLAIISNKDEDSFIR | 2250.1401 | 2250.1763 |
| MNYTNWSPGQPDNAGGIEHCLELR | 2773.2456 | 2773.2913 |
Mass fragments of tryptic peptides extracted after SDS-PAGE identified the 46.8 kDa protein as the full length His-tagged GFP-perlucin including the two C-terminal repeat motifs “DSLHANLQQR” and “NSLHANLQQR” (Figure 4E).