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. 2012 Oct 3;7(10):e46559. doi: 10.1371/journal.pone.0046559

Figure 5. Intracellular localization of SP-A2 wild-type and variants.

Figure 5

CHO-K1 Cells were transiently transfected with SP-A2 wild-type, G231V, F198S, Q223K plasmids, 48 h after transfection, the cells were fixed by 4% PFA, the localization of SP-A2 was examined by indirect immunofluorescence assay, and images were collected by confocal microscopy as described in Materials and Methods. Wild-type and Q223K distributed evenly in cellular which colocalized in part with calreticulin (ER marker) (H,T). Most of G231V, F198S mutants colocalized with calreticulin (L,P). Scale bar 10 µm.