Skip to main content
. 2012 Oct 3;7(10):e46831. doi: 10.1371/journal.pone.0046831

Figure 1. Effects of glucose and Zn2+ chelation by TPEN on Mt1a, Mt2a, ZnT1 and ZnT8 mRNA expression in overnight cultured rat islets.

Figure 1

Rat islets were precultured for 1 week in serum-free RPMI medium containing G10 and 5 g/l BSA. They were then cultured for 18 h in the presence of 5, 10 or 30 mmol/l glucose (G5, G10 or G30) with DMSO (open bars) or with 6.25 µmol/l TPEN (dissolved in DMSO) (closed bars). Mt1a, Mt2a, ZnT1 and ZnT8 to Tbp mRNA ratios were measured by real-time PCR. Except for Mt2a, the Gene to Tbp mRNA ratios were expressed relative to the ratio measured in islets cultured in G10 without TPEN. C, the mean Ct for Tbp was 27.5 in G5, 29.3 in G5+TPEN, 28 in G10 and 28.9 in G10+TPEN. D, the mean Ct for Tbp was 27.9 in G10, 28.8 in G10+TPEN, 27.8 in G30 and 29.2 in G30+TPEN. Results are means ± SEM for 3 experiments. *, P<0.05 for the effect of G5 vs. G10 and #, P<0.05 for the effect of TPEN by two-way ANOVA followed by a test of Bonferroni.