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. 2012 Oct 3;7(10):e46831. doi: 10.1371/journal.pone.0046831

Figure 4. Effects of glucose and ZnCl2 on the stimulation of rat islet cell apoptosis by prolonged culture in low and high glucose concentrations.

Figure 4

Rat islets were cultured for 3 days (A, D) or 1 week (B,C,E,F) in the presence of G5, G10 or G30±100 µmol/l ZnCl2 as indicated. A, B, C, DNA fragmentation was measured by an ELISA kit and expressed relative to the value measured in islets cultured in G10. D, E, F, the percentage of apoptotic β-cells was measured by TUNEL. Results are means ± SEM for 3–5 experiments. *, P<0.05 for the effect of G5 or G30 vs. G10 and #, P<0.05 for the effect of ZnCl2 by two-way ANOVA followed by a test of Bonferroni (A,B,C) or by one-way ANOVA followed by a test of Newman-Keuls (D,E,F).