Table 1. Summarized data of chimeric virus utilized in in vitro selections.
RT Origin | RT Subtype | RT fragment codons | Vectors used | Virus production methodology | Virus name | Replicates | Amino acids differences between RT subtype B and C |
NL4-3 | B | 35–225 | ΔDNA polimerase RT HXB2 (11) | recombination | RTB | 1 | Reference |
patient | C | 35–225 | ΔDNA polimerase RT HXB2 (11) | recombination | RTC | 1 | V35T, E36A, T39D, K43R, S48T, V90F, Q102R, D121Y, K122E, D123N, S134I, I135T, C162S, E169K, K173N, Q174K, D177E, T200A, Q207E, R211K, F214L |
NL4-3 | B | 25–554 | ΔRT HXB2 (13) | cloning | RTB′ | 6 | Reference |
C23 patient | C | 25–315 | ΔNRT HXB2 (14) | cloning | RTC′ | 6 | V35T, E36A, T39D, K43R, S48T, Q102K, D121Y, K122E, C162S, K173N, Q174K, D177E, T200A, Q207E, R211K |