Table 2. Drug resistance mutations found during the in vitro selection of RTB′ and C′ with escalating concentration of 3TC.
| 3TC | RTB′ (1) | RTB′ (2) | RTB′ (3) | RTB′ (4) | RTB′ (5) | RTB′ (6) | RTC′ (1) | RTC′ (2) | RTC′ (3) | RTC′ (4) | RTC′ (5) | RTC′ (6) |
| Time (days) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) | Mutation (µM) |
| 6 | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| 10 | WT (0.04) | NS | NS | WT (0.08) | NS | NS | WT (0.08) | (0.08) | (0.08) | WT (0.08) | NS | NS |
| 16 | NS | NS | NS | NS | NS | NS | M184M/I (0.16) | M184M/I(0.16) | M184M/I (0.16) | NS | NS | NS |
| 20 | WT (0.16) | WT (0.16) | WT (0.16) | WT (0.32) | WT (0.32) | WT (0.32) | M184M/I (0.32) | M184M/I (0.32) | M184M/I (0.32) | WT (0.32) | WT (0.32) | WT (0.32) |
| 25 | WT (0.32) | M184M/I (0.32) | M184M/I (0.32) | WT (0.64) | M184M/I (0.64) | M184M/I (0.64) | M184M/I (0.64) | M184M/I (0.64) | M184M/I(0.64) | M184M/I(0.64) | M184M/I (0.64) | |
| 31 | M184M/I (0.64) | M184M/I (0.64) | M184M/I (0.64) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) | M184M/I (1.28) |
| 38 | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| 44 | M184M/I (2.56) | M184M/I (2.56) | M184M/I (2.56) | M184M/I (5.12) | M184M/I (5.12) | M184M/I (5.12) | M184M/I (5.12) | M184M/I (5.12) | M184M/I (5.12) | M184M/I; E203E/K* (5.12) | M184M/I (5.12) | M184M/I (5.12) |
| 50 | M184M/I (5.12) | M184M/I (5.12) | M184I (5.12) | M184I (10.24) | M184I (10.24) | M184I (10.24) | M184I (10.24) | M184I (10.24) | M184I (10.24) | M184M/I (10.24) | M184M/I (10.24) | M184M/I (10.24) |
| 56 | M184I (10.24) | M184I (10.24) | M184I (10.24) | M184I (20.48) | M184I (20.48) | M184I (20.48) | M184I (20.48) | M184I (20.48) | M184I (20.48) | M184I; E203E/K* (20.48) | M184I (20.48) | M184M/I (20.48) |
| 62 | NS | NS | NS | NS | NS | M184I (40.96) | NS | NS | M184I (40.96) | NS | NS | M184M/I/V (40.96) |
| 69 | M184I (40.96) | NS | NS | M184I (81.92) | NS | M184I (81.92) | M184I (81.92) | NS | M184I (81.92) | M184I; E203E* (81.92) | NS | M184M/I/V (81.92) |
| 77 | NS | NS | NS | M184M/I/V (163.84) | NS | M184M/I/V (163.84) | NS | NS | M184I (163.84) | NS | NS | M184M/I/V (163.84) |
| 84 | M184I (163.84) | M184I (163.84) | M184I (163.84) | M184M/I/V (327.68) | M184I (327.68) | M184M/I/V (327.68) | M184I (327.68) | M184I (327.68) | M184V (327.68) | M184I; E203E/K* (327.68) | M184I (327.68) | M184V (327.68) |
| 90 | M184I (327.68) | M184I (327.68) | M184I (327.68) | M184M/I/V (655.36) | M184M/I/V (655.36) | M184M/I/V (655.36) | (655.36) | (655.36) | NS | M184I; E203E/K* (655.36) | M184M/I/V (655.36) | NS |
| 96 | M184M/I/V (655.36) | M184I (655.36) | M184I (655.36) | M184M/I/V (1310.72) | M184M/I/V (1310.72) | M184M/I/V (1310.72) | M184M/I/V (1310.72) | (1310.72) | NS | M184M/I/V; E203E/K* (1310.72) | M184M/I/V (1310.72) | NS |
| 104 | M184M/I/V (1310.72) | M184M/I/V (1310.72) | M184M/I/V (1310.72) | M184V (2621.44) | M184V (2621.44) | M184V(2621.44) | M184V (2621.44) | M184V (2621.44) | M184V(2621.44) | M184M/I/V; E203K* (2621.44) | M184V (2621.44) | M184V (2621.44) |
| 110 | M184M/I/V (2621.44) | M184M/I/V (2621.44) | M184M/I/V (2621.44) | M184V (5242.88) | M184V (5242.88) | M184V (5242.88) | M184V (5242.88) | M184V (5242.88) | M184V (5242.88) | M184V; E203K* (5242.88) | M184V (5242.88) | M184V (5242.88) |
NS - not sequenced,
*
- mutation not associated with 3TC resistance, () - replicate number.