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. 2012 Oct 3;7(10):e46298. doi: 10.1371/journal.pone.0046298

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© 2012 Kumar et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Presence of the UV traceable bis-aryl hydrazone bond verified by measuring absorbance of Ac1 and Ac2 samples in Nano-Drop spectrophotometer (ND-100) with unmodified actin sample (green) as control. Note, the comparatively noisy spectra result from a 1 mm optical path length in the Nano-drop spectrophotometer and multiplication by 10 before plotting to make data comparable to measurements using a conventional spectrophotometer with 1 cm path length. (B) Data similar to those in (A) but for Ac3, Ac4 and Ac5 conjugates. (C) Data for anti-human CD45 monoclonal antibody (MAb) conjugated with actin (AcM).