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. 2012 Oct 3;7(10):e46482. doi: 10.1371/journal.pone.0046482

Figure 4. KLF1 enhances DNaseI hypersensitivity site formation at the Alad1b promoter.

Figure 4

(A+B) DNaseI hypersensitivity assay in K1-ERp cells treated with vehicle control or 4-OHT at the Alad1b and β-globin promoters respectively. (C+D) ChIP performed on chromatin derived from K1-ERp cells treated with either vehicle control or 4-OHT. Antibodies were specific to (C) Brg1 or (D) histone H3. Target DNA enrichment relative to input was determined by Q-PCR using Alad1b or β-globin promoter primers. *p value≤0.05. **p value ≤0.01 by Student’s t-test. Data shown represents the average of at least 3 independent experiments (mean ± SEM).