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. 2012 Oct 3;7(10):e46386. doi: 10.1371/journal.pone.0046386

Figure 1. Chs3p residues 19DEESLL24 constitute an intracellular retention signal.

Figure 1

(A) Ten-fold serial dilutions of chs3Δ chs6Δ cells (TSY194) expressing point mutant alleles of CHS3 that define 19DEESLL24 as an intracellular retention signal. Dilutions were spotted on YPD, ½ YPD, SC-Ura (-Ura) and SC-Ura +50 ug/ml calcofluor (-Ura +CF). (B) Chitin staining of TSY194 expressing the chs3-L24P mutant allele. (C) Ten-fold serial dilutions of TSY194 expressing alanine-scanning point mutants of Chs3p residues 17–25.