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. 2012 Oct 3;7(10):e46386. doi: 10.1371/journal.pone.0046386

Figure 3. AP-3 is required for the intracellular retention of Chs3p.

Figure 3

(A) Ten-fold serial dilutions of wt (YPH499), chs6Δ (TSY49), chs6Δ apl2Δ (RSY3393), chs6Δ apl1Δ (TSY300) and chs6Δ apl6Δ (TSY269) cells on YPD, YPD + 50 ug/ml calcofluor (YPD + CF), synthetic complete medium (SC), and synthetic complete medium +50 ug/ml calcofluor (SC + CF). (B) An average of 5 quantitative chitin assays. Cultures were grown to saturation and cell walls were isolated by lysing 35–50 mg of cells in 6% KOH. Chitin was digested with chitinase from T. viride and the resulting GlcNAc was quantified. For each assay the results of 2–3 replicates of each strain were averaged and normalized against the wt level of chitin. Five separate normalized experiments were then averaged together.