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. 2012 Oct 3;7(10):e46386. doi: 10.1371/journal.pone.0046386

Table 2. Plasmids used in this study.

Name Description Source
pJC345 EcoRI-SalI genomic fragment containing CHS3 subcloned in pRS316 [50]
pCHS3QC1 W391R (Chs3W391R) mutation in pJC345 This study
pCHS3QC2 W391L (Chs3W391L) mutation in pJC345 This study
pCHS3QC3 W391F (Chs3W391F) mutation in pJC345 This study
pCHS3QC4 W391S (Chs3W391S) mutation in pJC345 This study
pCHS3QC5 W391A (Chs3W391A) mutation in pJC345 This study
pCHS3QC6 W391T (Chs3R374T) mutation in pJC345 This study
pCHS3QC7 W391A (Chs3R374A) mutation in pJC345 This study
pCHS3QC103 N17AQ18A (Chs3N17AQ18A) mutation in pJC345 This study
pCHS3QC133 D19A (Chs3D19A) mutation in pJC345 This study
pCHS3QC135 E20A (Chs3E20A) mutation in pJC345 This study
pCHS3QC137 E21A (Chs3E21A) mutation in pJC345 This study
pCHS3QC131 S22A (Chs3S22A) mutation in pJC345 This study
pCHS3QC106 L23A (Chs3L23A) mutation in pJC345 This study
pCHS3QC107 L24A (Chs3L24A) mutation in pJC345 This study
pCHS3QC139 R25A (Chs3R25A) mutation in pJC345 This study
pCHS3QC115 N17AQ18A (Chs3N17AQ18AW391R) mutation in pCHS3QC1 This study
pCHS3QC134 D19A (Chs3D19AW391R) mutation in pCHS3QC1 This study
pCHS3QC136 E20A (Chs3E20AW391R) mutation in pCHS3QC1 This study
pCHS3QC138 E21A (Chs3E21AW391R) mutation in pCHS3QC1 This study
pCHS3QC132 S22A (Chs3S22AW391R) mutation in pCHS3QC1 This study
pCHS3QC118 L23A (Chs3L23AW391R) mutation in pCHS3QC1 This study
pCHS3QC119 L24A (Chs3L24AW391R) mutation in pCHS3QC1 This study
pCHS3QC144 R25A (Chs3R25AW391R) mutation in pCHS3QC1 This study
pD15 chs3p deleted of N-terminal 15 AA (Δ15CHS3)subcloned in p416MET25 This study
pD15WR W391R (Δ15CHS3(W391R)) mutation in pD15 This study
pD25 chs3p deleted of N-terminal 25 AA (Δ25CHS3) subcloned in p416MET25 This study
pD25WR W391R (Δ25CHS3(W391R)) mutation in pD25 This study
pJC322 N-terminal 171AA of Chs3p GST-tagged (Chs3(1–170)) in pGEX-2T [50]
pCHS3QC164 D19AE21A (Chs3(1–170)D19AE21A) mutation in pJC322 This study
pCHS3-2GST Second cytosolic loop of Chs3p GST-tagged (Chs3(224–451)) in pGEX-2T This study
pTS11 L24H (Chs3-L24H) mutation in pJC345 This study
pTS13 L24F (Chs3-L24F) mutation in pJC345 This study
pTS15 L24P (Chs3-L24P) mutation in pJC345 This study
pTS18 D19N (Chs3-D19N) mutation in pJC345 This study
pTS166 DEESLLΔ (Chs3-DEESLLΔ) mutation in pJC345 This study