Figure 4. Pharmacokinetics analysis in single cells.

(A) Two-tissue compartmental model describing FDG pharmacokinetics, including influx (K ₁), efflux (k ₂), phosphorylation to FDG-6-phosphate (k ₃), and dephosphorylation (k ₄). (B) Patlak analysis modeling FDG influx kinetics for a single cell (highlighted by a red circle in Figure 3A). (C,D) Rate of efflux (k ₂) and phosphorylation (k ₃) plotted as a function of rate of influx (K ₁) for all the cells in the microscope’s field of view. (E) Compartmental analysis modeling FDG efflux kinetics from a single cell (highlighted by a red circle in Figure 3C) after withdrawal of FDG, presenting a fast and a slow component. (F) The model for FDG efflux is the sum of a fast and a slow component (rates λ ₁ and λ ₂, respectively), which are plotted for all the cells in the field of view.