Table 5.

Identification of proteins from cell surface or cellular location with affinity for α1(XI)NTD by affinity chromatography-mass spectrometry.

Protein namea	Common Abbreviationb	Mr Pred.c (kDa)	Mr Experimentald (kDa)	Peptide Matchese	Seq.cov.(%)f
Cell Surface Location
Annexin I	ANXA1	29.1	35, 32	9	39
Annexin II	ANXA2	49.4	38,35,32	14	40
Annexin V	ANXA5	36.1	35	16	62
Calreticulin (GRP60)	CALR	48	55	6	18
Nucleolin	NUCL	77.4	100	10	13
Protein Fate and Modification
Procollagen -lysine, 2-oxoglutarate 5-dioxygenase 1	PLOD1	83.5	75	8	16
Protein disulfide isomerase A3	PDIA3	56.7	60,55	17	39
Protein Disulfide Isomerase A4	PDIA4	72.5	75	8	18
Heat shock 70kDa protein 5(GRP78)	HSPA5	72.3	75	8	18
Tumor rejection antigen (gp96 or HSP90B1)	HSP90B1	92.4	100	6	9
Metabolic, cytoskeletal, and nuclear proteins
Fructose Bisphophate Aldolase	ALDOA	39.5	45,38	10	30
Serotransferrin	TF	77.8	75	8	12
Lactate dehydrogenase A	LDHA	36.6	32, 35	9	31
Actin, beta	ACTB	42	29	13	45
Alpha-actinin 4	ACTN4	104.8	100	9	15
Acidic leucine-rich nuclear phosphoprotein 32 family member B	ANP32B	29.9	32	5	15

^aFull name of reference sequence;

^bCommon protein abbreviation;

^cPredicted Mr according to NCBI;

^dExperimental Mr calculated by analysis of gel images;

^eNumber of peptide matches corresponding to the reference sequence;

^fSequence coverage calculated as the number of amino acids identified of the total number of amino acids in reference sequence.