Table 2.
Effects of Chronic Alcohol Exposure and Leptin Administration on Body Weight, Liver Weight, Epididymal WAT Weight, and Blood Metabolites
| Measurements | PF | AF | AF/Leptin |
|---|---|---|---|
| Food intake (g/d per mouse) | 10.36 ± 0.83 | 11.02 ± 0.76 | 10.67 ± 1.47 |
| Alcohol intake (g/d per mouse) | NA | 0.59 ± 0.04 | 0.56 ± 0.07 |
| Body weight (g) | 29.77 ± 1.76 | 24.05 ± 1.40⁎ | 22.60 ± 0.52⁎ |
| Liver weight (g) | 1.25 ± 0.08 | 1.30 ± 0.07 | 1.09 ± 0.05⁎ |
| Epididymal WAT weight (g) | 1.28 ± 0.21 | 0.42 ± 0.04⁎ | 0.18 ± 0.04⁎† |
| Liver–body weight ratio | 4.17 ± 0.36 | 5.41 ± 0.14⁎ | 4.83 ± 0.14⁎ |
| Epididymal WAT–body weight ratio | 4.27 ± 0.59 | 1.74 ± 0.12⁎ | 0.78 ± 0.18⁎† |
| Plasma ALT (U/mL) | 24.36 ± 7.19 | 65.82 ± 9.60⁎ | 31.50 ± 11.18† |
| Blood glucose (mg/dL) | 277.0 ± 45.4 | 221.0 ± 19.1⁎ | 204.7 ± 26.1⁎ |
| Blood ketone bodies (mg/dL) | 4.90 ± 1.59 | 7.08 ± 1.65⁎ | 8.34 ± 1.25⁎ |
| Plasma triglycerides (mg/dL) | 75.45 ± 14.12 | 76.98 ± 21.32 | 57.83 ± 20.40 |
| Plasma cholesterol (mg/dL) | 91.29 ± 17.88 | 59.81 ± 11.24⁎ | 47.85 ± 9.66⁎ |
| Plasma free fatty acids (mmol/L) | 0.80 ± 0.15 | 0.43 ± 0.21⁎ | 0.72 ± 0.21† |
Mice were pair-fed alcohol or isocaloric maltose dextran for 8 weeks in the presence or absence of external leptin for the last 2 weeks.
Data are given as mean ± SD (n = 6–10).
AF, alcohol-fed; ALT, alanine aminotransferase; ANOVA, analysis of variance; PF, pair-fed; WAT, white adipose tissue.
⁎
P < 0.05 versus PF (ANOVA).
†
P < 0.05 versus AF (ANOVA).