Table 3.
Effects of Leptin Administration on Hepatic Genes Related to Lipid Metabolism
| Measurements | PF | AF | AF/Leptin |
|---|---|---|---|
| Fatty acid transport | |||
| FABP1 | 1.006 ± 0.13 | 0.41 ± 0.13⁎ | 0.33 ± 0.09⁎ |
| FATP2 | 1.005 ± 0.12 | 0.98 ± 0.29 | 1.50 ± 0.29⁎† |
| FATP5 | 1.003 ± 0.10 | 0.98 ± 0.10 | 1.23 ± 0.17 |
| Fatty acid β-oxidation | |||
| CPT1a | 1.001 ± 0.05 | 0.53 ± 0.02⁎ | 1.06 ± 0.27† |
| ACADL | 1.009 ± 0.16 | 0.70 ± 0.15⁎ | 1.007 ± 0.11† |
| ACOX1 | 1.001 ± 0.05 | 0.58 ± 0.14⁎ | 0.86 ± 0.26† |
| VLDL assembly | |||
| MTTP | 1.04 ± 0.35 | 0.64 ± 0.09⁎ | 0.71 ± 0.11 |
| ApoB | 1.003 ± 0.10 | 0.76 ± 0.02⁎ | 1.17 ± 0.20† |
| Transcription factors | |||
| C/EBP-α | 1.004 ± 0.11 | 0.67 ± 0.02⁎ | 0.93 ± 0.08† |
| HNF-1α | 1.008 ± 0.15 | 0.56 ± 0.12⁎ | 0.84 ± 0.12† |
| HNF-4α | 1.01 ± 0.21 | 1.07 ± 0.15 | 1.27 ± 0.27 |
| PPAR-α | 1.004 ± 0.11 | 0.62 ± 0.09⁎ | 0.93 ± 0.05† |
| PPAR-γ | 1.007 ± 0.14 | 0.27 ± 0.16⁎ | 0.16 ± 0.06⁎ |
Mice were pair-fed alcohol on isocaloric maltose dextran for 8 weeks in the presence or absence of external leptin for the last 2 weeks.
Gene expression was analyzed using quantitative RT-PCR.
Data are given as mean ± SD (n = 4).
AF, alcohol-fed; ANOVA, analysis of variance; PF, pair-fed; VLDL, very low-density lipoprotein.
⁎
P < 0.05 versus PF (ANOVA).
†
P < 0.05 versus AF (ANOVA).