Figure 7.

Effect of cryptococcal PIK1, RUB1, and ENA1 deletion on macrophage activation profile in C. neoformans–infected lungs. mRNA was isolated from adherence-enriched pulmonary macrophages at week 3 postinfection and converted to cDNA. Arginase (ARG1) and nitric oxide synthase (iNOS) expression levels were determined as in Figure 6, and the ratio between Arg1/iNOS is shown as readout of M2 versus M1 macrophage activation status. Note the profound M2 bias in macrophages from H99-infected lungs, shift of the balance toward M1 in pik1Δ-, rub1Δ-, and ena1Δ-infected groups, with the ena1Δ group showing the most significant shift toward he M1 phenotype. n = 5 or more infected mice for each of the strains; mutant-infected groups significantly different from *H99- and ^†ena1Δ-infected groups.