Table 1.
Effect of Cryptococcal PIK1, RUB1, and ENA1 Deletions on the Extrapulmonary Dissemination of C. neoformans
| Organ and strain | Days post-infection |
Death | ||
|---|---|---|---|---|
| 7 | 14 | 21 | ||
| Spleen | ||||
| H99 | 0 | 1.23 ± 0.33 | 3.37 ± 0.34 | 3.37 ± 0.5 |
| pik1Δ | 0 | 0 | 0 | 3.58 ± 0.3 |
| rub1Δ | 0 | 0 | 0 | 2.42 ± 0.22⁎† |
| ena1Δ | 0 | 0 | 0 | |
| Brain | ||||
| H99 | 0.68 ± 0.43 | 2.48 ± 0.82 | 4.99 ± 0.94 | 5.92 ± 0.26 |
| pik1Δ | 0 | 0 | 0 | 5.61 ± 0.41 |
| rub1Δ | 0 | 0 | 0.33 ± 0.33 | 3.75 ± 0.52⁎† |
| ena1Δ | 0.32 ± 0.32 | 0 | 0 | |
Brains and spleens of the mice infected with pik1Δ, rub1Δ, ena1Δ mutants, and the wild-type strain H99 were harvested at days 7, 14, 21, postinfection and at death for analysis of fungal burden. Data are expressed as the mean log colony-forming units (CFU) ± SEM (5 or more mice per group at each time point).
⁎
P < 0.05 compared to H99 group.
†
P < 0.05 compared to pik1Δ group.