Figure 2.

iNOS mediates T-cell–induced crypt cell apoptosis downstream of TNFR1 and TNFR2. A: Fold induction of iNOS and TNF mRNA expression 3 hours after anti-CD3 injection determined by real-time RT-PCR from SB tissue from WT, TNFR1/2^−/−, and iNOS^−/− mice. B: WB analysis of iNOS protein expression in the indicated mice before, or 3 hours after, T-cell activation. β-Actin is used as a loading control. ND, not determined.