Figure 3.

iNOS is required for IEC apoptosis. A: Mice are sacrificed 24 hours after control or anti-CD3 mAb injection, and apoptosis is quantified in SB sections from WT mice, with or without L-NIL treatment, and from iNOS^−/− mice. Apoptotic indexes are calculated based on counting TUNEL-positive cells. ^⁎P < 0.001 compared with anti-CD3-treated WT mice. B: Activation of caspases 3, 8, and 9 is assessed by WB for the cleaved forms of the indicated protein in WT and iNOS^−/− mice at 0, 3, 6, or 12 hours after T-cell activation. Procaspase 3 is also shown, and β-actin is used as a loading control.