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. 2012 Oct;181(4):1250–1270. doi: 10.1016/j.ajpath.2012.06.036

Figure 1.

Figure 1

A motogenic response to HA fragments requires RHAMM. A: RHAMM binds to 220-kDa and 10-kDa HA. Lysates from RHAMM overexpressing mesenchymal cells were incubated with 220-kDa and 10-kDa HA coupled to Sepharose beads. Proteins bound to beads were resolved on SDS-PAGE, and RHAMM was detected by Western blot analysis. Arrows indicate bands that represent full-length RHAMM (RHAMMFL) and a processed (truncated) RHAMMTR that is commonly expressed after an injury. B: Wild-type and RHAMM−/− MEFs were seeded sparsely on tissue culture plastic and serum starved in defined medium. Cell migration was stimulated with 10% FCS (positive control), 220-kDa HA, or 10-kDa HA and quantified by real-time cinemicrography. Values represent mean ± SD of 50 cells. *P < 0.001 between wild-type and RHAMM−/− cells, †P < 0.001 versus defined medium.