Figure 7.

P15-1 inhibits collagen I accumulation within wounds. A: Full-thickness excisional wounds were treated with collagen vehicle alone (0 μg/mL P15-1 concentration) or P15-1 + collage vehicle (25, 100 μg/wound site). At day 7, wounds were isolated, and the OH-proline content was analyzed as described in Materials and Methods. P15-1 significantly reduced wound collagen at day 7 after wounding at both concentrations (*P < 0.01). Each column represents the mean ± SD. n = 6. B: Full-thickness excisional wounds were treated with either collagen vehicle alone or P15-1 in collagen vehicle (100 ng/mL to 1 μg/wound site). Wound tissue mRNA was isolated 24 hours after wounding, and collagen I transcripts were amplified by RT PCR. Amplification of β-actin transcripts was used as loading control. Amplification products were separated on an agarose gel and quantified by densitometry. The collagen I amplification products were corrected for equal loading by calculating the ratio collagen I/β-actin control. Collagen I expression in control wounds were set to one. P15-1 decreased collagen I mRNA expression 24 hours after wounding. The values represent typical results that were replicated three separate times.