Figure 8.

Peptide 15-1 reduces in vitro collagen production of cultured dermal fibroblasts. Monolayers of rat dermal fibroblasts were scratched multiple times using a plastic comb. A: Cells were incubated for the indicated times in medium containing either peptide 15-1 (30 μg/mL) or scrambled control peptide (Scr Pep; 30 μg/mL). B: Collagen I production was quantified by Western blot analysis. β-actin was used as loading control. Protein band intensities were determined by densitometry, and the ratio of Col I/β-actin was calculated. P15-1 detectably reduced collagen I protein levels at 24, 48, and 72 hours after its addition. Values represent the mean ± SD. n = 3. *P < 0.001.