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TIMP-1 and COLIA1 expression in cultured human VSMCs treated with 5-Aza for 48 hours. A: The fold change in the expression for TIMP1 and COLIA1 is not significantly changed by 5-Aza treatment compared with control (n = 8). B: TIMP-1 protein levels in media are not significantly changed compared with controls (n = 4). C: Western blot analysis for pro-COLIA1, and COLIA1 demonstrates no effect of 5-Aza treatment on the protein expression of COLIA1 (n = 3). Data are shown as mean ± SEM.
