Table 3.

Kinetics of PK activation

	PK autoactivation		PK activation by FXIIa
Activating surface	k2,app (+HK)	k2,app (−HK)	rate (+HK)	rate (−HK)
	× 105 M−1s−1	× 105 M−1s−1	nM·min−1nM−1	nM·min−1nM−1
dextran sulfate	2.9 ± 0.25	NAa	445 ± 87	374 ± 58
Ni-NTA-liposomes	2.8 ± 0.04	NA	545 ± 43	366 ± 67
NTA-liposomes	1.0 ± 0.10	NA	310 ± 20	348 ± 45
No surface	NA	NA	29 ± 9	126 ± 65

Data are mean ± SEM (n = 3).

^aNA, negligible activity.