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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Clin Cancer Res. 2012 Aug 6;18(19):5364–5373. doi: 10.1158/1078-0432.CCR-12-0961

Figure 3. SCH 900776 enhances cytarabine-induced apoptosis in human AML cell lines.

Figure 3

A, HL-60 cells were treated for 24 h with diluent (0.2% DMSO), 300 nM cytarabine, 300 nM SCH 900776 or 300 nM cytarabine + 300 nM SCH 900776. After staining with PI, flow cytometry was performed as indicated in the METHODS. B, percentage of events with <2n DNA content calculated from histograms in panel A and additional samples from the same experiment. C, HL-60 cells were treated for 24 h with the indicated concentrations of cytarabine in the presence of diluent (lanes 1, 5, 9 and 13) or SCH 900776 at 100 (lanes 2, 6, 10 and 14), 300 (lanes 3, 7, 11 and 15) or 1000 nM (lanes 4, 8, 12 and 16). Samples in lanes 13–16 also contained the caspase inhibitor Q-VD-OPh at 5 μM. At the completion of the incubation, cells were lysed in 6 M guanidine hydrochloride under reducing conditions, prepared for SDS-PAGE and subjected to immunoblotting with antibodies to the indicated antigens. D, percentage of subdiploid events observed with ML-1 treated for 24 h with the indicated concentrations of cytarabine and SCH 900776.