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LPS treatment enhanced SCOT nitration in rat kidney. Forty micrograms of the high-speed supernatant fractions was loaded per lane. Proteins transferred to the PVDF membrane were analyzed with Upstate Biotechnology monoclonal anti-nitrotyrosine antibody (A) or polyclonal anti-SCOT antibody (B). Lanes 1–5 correspond to 0, 6, 9, 16, and 24 h after LPS injection, respectively.
