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(A) Stacked mean absorbance spectra (overlay shown in the inset), (B) overlay mean second-derivative spectra (nine-point Savitzky-Golay smoothing window), and (C) stacked second-derivative spectra of unfixed (dried) cells 24 h (red), 1 week (green), 2 weeks (black), and 4 weeks (blue) after sample preparation.
