Figure 1.
1H-NMR end-point assay of HppE with (R)- and (S)-7. (A) Initial time point recorded 3 min after incubation of substrate with enzyme. (B) 36 min time point showing >90% conversion of (S)-7 to 13. (C) 36 min time point showing >90% conversion of (R)-7 to 15. (See Figure S4-1, S4-2 for details).