A, Immunohistochemical staining shows increased expression of PI3K p110 subunit, p-Akt, p-S6, and survivin in Tgfbr1/Pten 2cKO mouse HNSCC (high magnification in the right panel), compared with Tgfbr1/Pten 2cKO mouse mucosa and Tgfbr1flox/flox/Ptenflox/flox mucosa (Scale Bars=50um). B, The quantification of immunostaining shows statistics by histoscore (Mean ± SEM,*, P < 0.05; **, P < 0.01;***, P <0.001, One-way ANOVA analysis, Tg, tongue; TSCC, tongue squamous cell carcinoma). C, Western blot shows a significant increase in p-Akt, p-mTOR, p-P70S6K, p-S6, p-4E-BP1, and survivin in Tgfbr1/Pten 2cKO mice HNSCC compared with Tgfbr1flox/flox/Ptenflox/flox tongue.