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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):447–456. doi: 10.1002/jcp.24151

Figure 7. LfcinB exerts anti-catabolic and anti-inflammatory effects in synovial fibroblasts.

Figure 7

(A) Synovial fibroblasts in monolayer were stimulated by IL-1β (50 pg/mL), in the presence or absence of LfcinB (50 and 100 μg/mL), for 24 hours. Then qPCR was performed to quantify MMP-1, MMP-3, and MMP-13 transcripts. In parallel, immunoblotting was carried out to assess the intracellular protein levels of MMP-1, MMP-3, and MMP-13. GADPH was used as loading controls. (B) Synovial fibroblasts were incubated with IL-1β (50 pg/mL), in the presence or absence of LfcinB (50 and 100 μg/mL), for 24 hours. The mRNA levels of IL-1β and IL-8 were measured by qPCR. (C) Synovial fibroblasts were treated with IL-1β (50 pg/mL), in the presence or absence of LfcinB (50 and 100 μg/mL), for 24 hours, followed by qPCR analyses of TLR2 transcripts. (D) Synovial fibroblasts were incubated with IL-1β (50 pg/mL), in the presence or absence of LfcinB (50 and 100 μg/mL), for 24 hours. The mRNA levels of iNOS were quantified by qPCR. (E) Synovial fibroblasts were plated on 96-well plates at 1×105 cells/well. Then cells were incubated with LfcinB (10, 50 and 100 μg/mL) for 24 hours. Each condition was set up in quadruplet. Assays were performed using CellTiter 96 AQueous One Solution. *p<0.05; **p<0.01.