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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):447–456. doi: 10.1002/jcp.24151

Table 1. qPCR primer sequences.

Gene Primer Seq. (5′→3′) NCBI
Reference No.
Annealing
Tm
18S rRNA F: CGGCTACCACATCCAAGGAA
R: GCTGGAATTACCGCGGCT
NR_003286.2 60°C
MMP1 F: AGTGACTGGGAAACCAGATGCTGA
R: GCTCTTGGCAAATCTGGCGTGTAA
NM_002421.3 60 °C
MMP3 F: GCGTGGATGCCGCATATGAAGTTA
R: AAACCTAGGGTGTGGATGCCTCTT
NM_002422.3 60 °C
MMP13 F:ACCCTGGAGCACTCATGTTTCCTA
R:TGGCATCAAGGGATAAGGAAGGGT
NM_002427.3 60 °C
ADAMTS4 F: ACTGGGCTACTACTATGTGCTGGA
R: CTTCTTCTTGGAGCCAATGATGCG
NM_005099.4 60 °C
ADAMTS5 F:CTGTGACGGCATCATTGGCTCAAA
R:TTCAGGAATCCTCACCACGTCAGT
NM_007038.3 60 °C
INOS F: ATCACACGCCCACAGAGATCCA
R:GCTTCAGGCTGTTGAGCCATGT
NM_000625.4 55 °C
TLR2 F: AAACGTTAACAATCCGGAGGCTGC
R:
TGTTGTGAAAGTAAACAAGGAACCAG
NM_003264.3 60 °C
IL1B F: ATGACCTGAGCACCTTCTTTCCCT
R: GCATCGTGCACATAAGCCTCGTTA
NM_000576.2 55 °C
IL6 F: AAGCCAGAGCTGTGCAGATGAGTA
R: TTCGTCAGCAGGCTGGCATTTGT
NM_000600.3 60 °C
IL8 F: TCTTGGCAGCCTTCCTGATTTCTG
R: GGGTGGAAAGGTTTGGAGTATGTC
NM_000584.3 60 °C
IL4 F: GCCTCACATTGTCACTGCAAATCG
R: AGGTGATATCGCACTTGTGTCCGT
NM_000589.2 60 °C
IL10 F: TTAAGGGTTACCTGGGTTGCCAAG
R: AGTTCACATGCGCCTTGATGTCTG
NM_000572.2 60 °C