Table 1. qPCR primer sequences.
Gene | Primer Seq. (5′→3′) | NCBI Reference No. |
Annealing Tm |
---|---|---|---|
18S rRNA | F: CGGCTACCACATCCAAGGAA R: GCTGGAATTACCGCGGCT |
NR_003286.2 | 60°C |
MMP1 | F: AGTGACTGGGAAACCAGATGCTGA R: GCTCTTGGCAAATCTGGCGTGTAA |
NM_002421.3 | 60 °C |
MMP3 | F: GCGTGGATGCCGCATATGAAGTTA R: AAACCTAGGGTGTGGATGCCTCTT |
NM_002422.3 | 60 °C |
MMP13 | F:ACCCTGGAGCACTCATGTTTCCTA R:TGGCATCAAGGGATAAGGAAGGGT |
NM_002427.3 | 60 °C |
ADAMTS4 | F: ACTGGGCTACTACTATGTGCTGGA R: CTTCTTCTTGGAGCCAATGATGCG |
NM_005099.4 | 60 °C |
ADAMTS5 | F:CTGTGACGGCATCATTGGCTCAAA R:TTCAGGAATCCTCACCACGTCAGT |
NM_007038.3 | 60 °C |
INOS | F: ATCACACGCCCACAGAGATCCA R:GCTTCAGGCTGTTGAGCCATGT |
NM_000625.4 | 55 °C |
TLR2 | F: AAACGTTAACAATCCGGAGGCTGC R: TGTTGTGAAAGTAAACAAGGAACCAG |
NM_003264.3 | 60 °C |
IL1B | F: ATGACCTGAGCACCTTCTTTCCCT R: GCATCGTGCACATAAGCCTCGTTA |
NM_000576.2 | 55 °C |
IL6 | F: AAGCCAGAGCTGTGCAGATGAGTA R: TTCGTCAGCAGGCTGGCATTTGT |
NM_000600.3 | 60 °C |
IL8 | F: TCTTGGCAGCCTTCCTGATTTCTG R: GGGTGGAAAGGTTTGGAGTATGTC |
NM_000584.3 | 60 °C |
IL4 | F: GCCTCACATTGTCACTGCAAATCG R: AGGTGATATCGCACTTGTGTCCGT |
NM_000589.2 | 60 °C |
IL10 | F: TTAAGGGTTACCTGGGTTGCCAAG R: AGTTCACATGCGCCTTGATGTCTG |
NM_000572.2 | 60 °C |