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Deletion of the J proteins Caj1, Djp1, Xdj1 and Hlj1 has little or no effect in the presence of compounds tested. A) Five-fold dilutions of Δcaj1, Δdjp1, Δxdj1, or Δhlj1 were plated on rich medium containing DMSO or compound and grown for 72 hours at 30°C. B,C) Strains were grown in liquid YPD in the presence of wortmannin (24–0 μg/mL) or hygromycin B (120–0 μg/mL) in DMSO (final concentration 2%).
