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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Clin Cancer Res. 2012 Aug 14;18(19):5352–5363. doi: 10.1158/1078-0432.CCR-12-0628

Figure 3. CSPG4-specific mAb TP41.2 reduces migration and invasion of MM cells.

Figure 3

A. PPM-Mill and Hmeso MM cells were pre-incubated with mAb TP41.2 or with IgG control for 1 hour. Cells were then plated on the membrane of the upper chamber of a Transwell® plate and grown for 48 hours in the presence of 10% FBS. Cells grown in serum-free conditions were used as a negative control. The cells migrated to the lower surface of the membrane were stained with Giemsa staining for microscopical observation. Then cells were solubilized and the absorbance was measured at 560nm to determine the extent of cell migration. The graph indicates the mean ± S.D. from three separate experiments. The statistical differences represent comparisons versus untreated cells using Students’s t test. P ≤ 0.05. B. PPM-Mill, Phi and REN MM cells were pre-incubated with mAb TP41.2 or with IgG control for 1 hour. Cells were then plated on the membrane of the upper chamber of a Matrigel®-coated Transwell® plate and grown for 48 hours in the presence of 10% FBS. Cells grown in serum-free conditions were used as a negative control. The cells migrated to the lower surface of the membrane were stained with Giemsa for microscopical observation. The graph indicates the mean ± S.D. from three separate experiments. The statistical differences represent comparisons versus untreated cells using Student’s t test. P ≤ 0.05.