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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Cancer Res. 2012 Aug 8;72(19):4944–4953. doi: 10.1158/0008-5472.CAN-12-1629

Figure 1.

Figure 1

The Sleeping Beauty transposon system. SB consists of two components, the transposable element (transposon) and the transposase enzyme, which catalyzes transposon mobilization. A, Vector for expressing the transposase under control of a tissue-specific promoter/enhancer. B, The SB transposon contains a pair of inverted repeat/direct repeat elements (IRDR), flanking the mobile cargo sequence. For cancer gene identification, the cargo sequence is designed to mimic retroviral insertional mutagenesis. The transposon contains splice acceptor sites (SA) and polyadenylation (pA) sequences to disrupt the expression of genes into which the transposon integrates (mRNA1). The transposon also contains 5’ sequences from the murine stem cell virus (MSCV) long terminal repeat (LTR) to serve as promoter/enhancer elements, which increase expression of adjacent genes (mRNA2). The MSCV LTR is followed by a splice donor (SD). Thus, a transcript initiated in the LTR can splice into downstream exons of endogenous genes. The SB transposon schematized here is the T2/Onc vector [reviewed in (16)].