Fig. 6.

The effect of P1/P2-depletion on ribosome depurination by RTA and PAP in vitro. (A) WT and P1/P2-depleted human ribosomes (2 pmol) were treated with RTA (0.1nM) for a time course 0–10 minutes. (B) WT and P1/P2-depleted ribosomes (2 pmol) treated with either RTA (0.1nM) or PAP (0.1 nM) for 3 minutes. rRNA was extracted, converted to cDNA, and depurination was measured by qRT-PCR using the comparative CT method (ΔΔCT). Data indicate the fold increases in depurination relative to untreated (no toxin) WT ribosomes and represent the mean ± SD of triplicate reactions from two independent experiments. * indicates P < 0.05 and N.S. indicates difference was not significant (P > 0.05), as analyzed by a Student’s unpaired, two-tailed t test.