Fig. 2.

Residual heterozygosity in distributable lines. The figure shows two chromosomes from line OR3252 (shown in Fig. 1) to illustrate the identification of segregating regions in distributable lines. The figure follows the conventions detailed in Fig. 1, with the top part of each subheading representing the contribution of the eight CC parental strains to the genome of the two most recent obligate ancestors and the midsection and lower sections representing the haplotypes of the line as provided in the CC website as figure or as text, respectively. a Chromosome 12 illustrates two segregating regions in which one of the most recent ancestors is homozygous while the other is segregating. The figure also illustrates that in some cases the most recent obligate ancestors may appear to have slightly different boundaries between parental contributions. We suggest that investigators rely on the haplotype reconstruction provided in the text file rather than on visual inspection of most recent ancestors. We expect these discrepancies to be resolved in the near future with use of MegaMUGA. b Chromosome 1 illustrates a segregating region in which each of the most recent ancestors’ parents was homozygous for a different parental allele