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. 2012 Sep 28;47(6):954–969. doi: 10.1016/j.molcel.2012.07.021

Figure 2.

Figure 2

Shutdown Is Required for the Biogenesis of All Ovarian piRNA Populations

(A) Shown are normalized levels of indicated ovarian small RNAs from shu[1] heterozygous and shu[1]/shu[def] flies.

(B) To the left, length profiles of normalized small RNAs split into miRNAs (small panels) and remaining RNAs (siRNAs and piRNAs) are shown. To the right, length profiles of repeat derived small RNAs (red antisense; blue sense) are shown; top panels show shu heterozygotes, bottom panels shu mutants.

(C) Scatter plot (log scale) showing levels of antisense piRNAs mapping to soma dominant (green), intermediate (yellow) or germline dominant (black) TEs in shu het. or shu mut. libraries.

(D) normalized piRNA profiles (sense up; antisense down) from shu het. (black) or shu mut. (red) libraries mapping to indicated TEs.

(E) normalized profiles of genome unique piRNAs (sense up; antisense down) from shu het. (black) or shu mut. (red) libraries mapping to indicated piRNA clusters (200nt walking window).