Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 May;79(10):3203–3207. doi: 10.1073/pnas.79.10.3203

Human platelet-derived growth factor stimulates amino acid transport and protein synthesis by human diploid fibroblasts in plasma-free media.

A J Owen 3rd, R P Geyer, H N Antoniades
PMCID: PMC346383  PMID: 6954470

Abstract

Purified human platelet-derived growth factor (PDGF) induces an increase in amino acid uptake via system A in quiescent human diploid fibroblasts. Cells must be exposed to PDGF for 45 min to obtain maximum transport stimulation. Transport stimulation requires protein synthesis. Transient exposure to PDGF, alone, in the absence of plasma components can stimulate transport. Acid-insoluble [3H]leucine incorporation is also stimulated by PDGF treatment, and this event also does not require the presence of plasma components. Finally, antiserum to PDGF that blocks PDGF-stimulated DNA synthesis in these cells also blocks PDGF-stimulated amino acid uptake and protein synthesis. Increased amino acid uptake and protein synthesis that occur soon after addition of fresh serum to quiescent cells can be attributed to the action of PDGF acting alone and should be useful as markers for the investigation of early cellular events caused by PDGF.

Full text

PDF
3203

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antoniades H. N. Human platelet-derived growth factor (PDGF): purification of PDGF-I and PDGF-II and separation of their reduced subunits. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7314–7317. doi: 10.1073/pnas.78.12.7314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Antoniades H. N., Scher C. D., Stiles C. D. Purification of human platelet-derived growth factor. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1809–1813. doi: 10.1073/pnas.76.4.1809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Busch C., Wasteson A., Westermark B. Release of a cell growth promoting factor from human platelets. Thromb Res. 1976 Apr;8(4):493–500. doi: 10.1016/0049-3848(76)90227-9. [DOI] [PubMed] [Google Scholar]
  4. Chait A., Ross R., Albers J. J., Bierman E. L. Platelet-derived growth factor stimulates activity of low density lipoprotein receptors. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4084–4088. doi: 10.1073/pnas.77.7.4084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen H. N. Exploiting amino acid structure to learn about membrane transport. Adv Enzymol Relat Areas Mol Biol. 1979;49:41–101. doi: 10.1002/9780470122945.ch2. [DOI] [PubMed] [Google Scholar]
  6. Clemmons D. R., Van Wyk J. J., Pledger W. J. Sequential addition of platelet factor and plasma to BALB/c 3T3 fibroblast cultures stimulates somatomedin-C binding early in cell cycle. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6644–6648. doi: 10.1073/pnas.77.11.6644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davies P. F., Ross R. Mediation of pinocytosis in cultured arterial smooth muscle and endothelial cells by platelet-derived growth factor. J Cell Biol. 1978 Dec;79(3):663–671. doi: 10.1083/jcb.79.3.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deuel T. F., Huang J. S., Proffitt R. T., Baenziger J. U., Chang D., Kennedy B. B. Human platelet-derived growth factor. Purification and resolution into two active protein fractions. J Biol Chem. 1981 Sep 10;256(17):8896–8899. [PubMed] [Google Scholar]
  9. Foster D. O., Pardee A. B. Transport of amino acids by confluent and nonconfluent 3T3 and polyoma virus-transformed 3T3 cells growing on glass cover slips. J Biol Chem. 1969 May 25;244(10):2675–2681. [PubMed] [Google Scholar]
  10. Guidotti G. G., Borghetti A. F., Gazzola G. C. The regulation of amino acid transport in animal cells. Biochim Biophys Acta. 1978 Dec 15;515(4):329–366. doi: 10.1016/0304-4157(78)90009-6. [DOI] [PubMed] [Google Scholar]
  11. Heldin C. H., Wasteson A., Westermark B. Growth of normal human glial cells in a defined medium containing platelet-derived growth factor. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6611–6615. doi: 10.1073/pnas.77.11.6611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heldin C. H., Westermark B., Wasteson A. Platelet-derived growth factor. Isolation by a large-scale procedure and analysis of subunit composition. Biochem J. 1981 Mar 1;193(3):907–913. doi: 10.1042/bj1930907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heldin C. H., Westermark B., Wasteson A. Platelet-derived growth factor: purification and partial characterization. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3722–3726. doi: 10.1073/pnas.76.8.3722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hershko A., Mamont P., Shields R., Tomkins G. M. "Pleiotypic response". Nat New Biol. 1971 Aug;232(33):206–211. [PubMed] [Google Scholar]
  15. Kaplan D. R., Chao F. C., Stiles C. D., Antoniades H. N., Scher C. D. Platelet alpha granules contain a growth factor for fibroblasts. Blood. 1979 Jun;53(6):1043–1052. [PubMed] [Google Scholar]
  16. Kohler N., Lipton A. Platelets as a source of fibroblast growth-promoting activity. Exp Cell Res. 1974 Aug;87(2):297–301. doi: 10.1016/0014-4827(74)90484-4. [DOI] [PubMed] [Google Scholar]
  17. Nakamura K. D., Weber M. J. Amino acid transport in normal and Rous sarcoma virus-transformed chicken embryo fibroblasts. J Cell Physiol. 1979 Apr;99(1):15–22. doi: 10.1002/jcp.1040990103. [DOI] [PubMed] [Google Scholar]
  18. Oxender D. L., Lee M., Cecchini G. Regulation of amino acid transport activity and growth rate of animal cells in culture. J Biol Chem. 1977 Apr 25;252(8):2680–2683. [PubMed] [Google Scholar]
  19. Pledger W. J., Stiles C. D., Antoniades H. N., Scher C. D. Induction of DNA synthesis in BALB/c 3T3 cells by serum components: reevaluation of the commitment process. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4481–4485. doi: 10.1073/pnas.74.10.4481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Quinlan D. C., Hochstadt J. The regulation by fibroblast growth factor of early transport changes in quiescent 3T3 cells. J Cell Physiol. 1977 Nov;93(2):237–246. doi: 10.1002/jcp.1040930209. [DOI] [PubMed] [Google Scholar]
  21. ROBINSON J. H., SMITH J. A. Density-dependent regulation of proliferation rate in cultured, androgen-responsive, tumour cells. J Cell Physiol. 1976 Sep;89(1):111–122. doi: 10.1002/jcp.1040890111. [DOI] [PubMed] [Google Scholar]
  22. Ross R., Glomset J., Kariya B., Harker L. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1207–1210. doi: 10.1073/pnas.71.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ross R., Vogel A. The platelet-derived growth factor. Cell. 1978 Jun;14(2):203–210. doi: 10.1016/0092-8674(78)90107-1. [DOI] [PubMed] [Google Scholar]
  24. Rutherford R. B., Ross R. Platelet factors stimulate fibroblasts and smooth muscle cells quiescent in plasma serum to proliferate. J Cell Biol. 1976 Apr;69(1):196–203. doi: 10.1083/jcb.69.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Scher C. D., Shepard R. C., Antoniades H. N., Stiles C. D. Platelet-derived growth factor and the regulation of the mammalian fibroblast cell cycle. Biochim Biophys Acta. 1979 Aug 10;560(2):217–241. doi: 10.1016/0304-419x(79)90020-9. [DOI] [PubMed] [Google Scholar]
  26. Slayback J. R., Cheung L. W., Geyer R. P. Comparative effects of human platelet growth factor on the growth and morphology of human fetal and adult diploid fibroblasts. Exp Cell Res. 1977 Dec;110(2):462–466. doi: 10.1016/0014-4827(77)90313-5. [DOI] [PubMed] [Google Scholar]
  27. Stiles C. D., Isberg R. R., Pledger W. J., Antoniades H. N., Scher C. D. Control of the Balb/c-3T3 cell cycle by nutrients and serum factors: analysis using platelet-derived growth factor and platelet-poor plasma. J Cell Physiol. 1979 Jun;99(3):395–405. doi: 10.1002/jcp.1040990314. [DOI] [PubMed] [Google Scholar]
  28. Vaitukaitis J., Robbins J. B., Nieschlag E., Ross G. T. A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab. 1971 Dec;33(6):988–991. doi: 10.1210/jcem-33-6-988. [DOI] [PubMed] [Google Scholar]
  29. Villereal M. L., Cook J. S. Regulation of active amino acid transport by growth-related changes in membrane potential in a human fibroblast. J Biol Chem. 1978 Nov 25;253(22):8257–8262. [PubMed] [Google Scholar]
  30. Vogel A., Raines E., Kariya B., Rivest M. J., Ross R. Coordinate control of 3T3 cell proliferation by platelet-derived growth factor and plasma components. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2810–2814. doi: 10.1073/pnas.75.6.2810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Weinstein R., Stemerman M. B., Maciag T. Hormonal requirements for growth of arterial smooth muscle cells in vitro: and endocrine approach to atherosclerosis. Science. 1981 May 15;212(4496):818–820. doi: 10.1126/science.7013068. [DOI] [PubMed] [Google Scholar]
  32. Westermark B., Wasteson A. A platelet factor stimulating human normal glial cells. Exp Cell Res. 1976 Mar 1;98(1):170–174. doi: 10.1016/0014-4827(76)90476-6. [DOI] [PubMed] [Google Scholar]
  33. Witte L. D., Kaplan K. L., Nossel H. L., Lages B. A., Weiss H. J., Goodman D. S. Studies of the release from human platelets of the growth factor for cultured human arterial smooth muscle cells. Circ Res. 1978 Mar;42(3):402–409. doi: 10.1161/01.res.42.3.402. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES