Figure 6.

HOIP RING2 and LDD are essential for NF-κB pathway activation. Dual Luciferase reporter assay for NF-κB activation. Full-length HOIP wild-type or HOIP cysteine mutants were co-expressed together with HOIL-1L (see lower panel) and a luciferase reporter construct, containing five NF-κB binding sites. A luciferase renilla construct was used as transfection control. Firefly luciferase values were normalized to renilla luciferase values. Normalized luciferase activity of 5 × NF-κB reporter vector (upper panel) is shown as mean±s.e.m. (*P<0.001, Student’s t-test, representative experiment of n=4).