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. 2012 Aug 28;31(19):3935–3948. doi: 10.1038/emboj.2012.237

Figure 4.

Figure 4

Impact of the arrangement of termination motifs on termination. (A) Sequence of the terminator variants analysed. (B) Northern blot analysis of RNAs derived from the constructs indicated. Read-through transcripts (RT) are indicated by a black arrow. (C) Northern blot analysis of constructs 78-wt and 78-INV upon metabolic depletion of the different components of the NNS complex. Cells were grown on glucose for 6 h (to deplete Nrd1p) or 14 h (to deplete Nab3p and Sen1p). Detection of the transcripts expressed from PTet is shown on the top. Expression of the NEL025c CUT (bottom) was monitored to verify the correct depletion of the proteins. The small RNA U4 was used as a loading control.