Figure 2.

Wg from the ISC/EB population but not the VM is required for ISC proliferation during regeneration. (A) Quantification of pH3^+ve cells from posterior midguts of the indicated genotypes (wg-IR and wg-IR^KK are independent RNAi transgenes for wg) after Sucrose (Suc) or bacterial (Pe) feeding. (B–I) Immunofluorescence of midguts as in (A) stained with anti-GFP (green) to visualize esg^+ve cells and Dapi (blue) to label all cell nuclei. (J–N) Quantification of pH3^+ve cells in posterior midguts of the indicated genotypes. Note that only RNAi for Wg or Wls involving the esg^+ve lineage (L), or heterozygosity for the null allele wg^CX4 (M) significantly inhibits ISC proliferation in response to damage. In contrast, no significant effect was seen by wg knockdown in the VM (J, K) or the ECs (N) (***P<0.0001 one-way ANOVA with Bonferroni’s multiple comparison test). Scale bars: 20 μm.