Figure 7.

JNK activation induces Wg in the Drosophila adult midgut. (A–D′) Control midguts (A, A′) or after forced activation of JNK in ISCs/EBs via activated (B, B′) or wild-type (C, C′) JNKK hemipterous (hep) for the indicated times and stained with anti-Wg antibody (red in left panels and grey in right ones). JNK activation resulted in drastic increase in the levels of Wg as assessed by staining (compare B–C′ with A, A′). (D) Forced activation of JNK in ECs labelled with MyoIA^ts>gfp (D; green) resulted in Wg upregulation in non-MyoIA expressing cells (arrows in D, D′). (E) qPCR quantification of wg, myc and puckered (puc) transcript levels in control midguts and midguts overexpressing activated hemipterous under the esg^ts>gfp driver (esg^ts>hep^act). (F) qPCR quantification of puckered (puc) transcript levels in control midguts and midguts overexpressing Wg under the esg^ts>gfp driver. (G) Quantification of pH3^+ve in the genotypes indicated. Note that knockdown of wg or Tcf repressed JNK-driven ISC proliferation (***P<0.0001 one-way ANOVA with Bonferroni’s multiple comparison test). Scale bars: 20 μm.