Table 1.
DNA-binding specificity scores
| Variant | Single substitution |
K range |
Overall preference |
|---|---|---|---|
| REDV | 510 | 1,100 | 920 |
| LRHN | 40 | 89 | 74 |
| RGPD | 38 | 75 | 65 |
| Wild type | 31 | 78 | 66 |
| KASN | 2.8 | 5.1 | 3.2 |
A mean K
was calculated for the set of sequences consisting of all possible single base-pair substitutions in the central 3 bp of the Zif268 DNA-binding site of the sequence with the highest binding affinity. The single substitution score is defined as this mean K
, divided by the K
of the sequence with the highest binding affinity. The K
range is defined as the K
of the sequence with the lowest DNA-binding affinity, divided by the K
of the sequence with the highest DNA-binding affinity. The K
range serves as a measure of the specificity of a variant for the sequence with the highest binding affinity versus the sequence it binds most weakly. The overall preference of a variant is defined as the mean K
of the set of all the sequences on the microarray except for the one with the highest binding affinity, divided by the K
of the sequence with the highest binding affinity.