Figure 1.

Episomal replication activity of 20mer constructs in transformed and normal cells. Histogram plot of the number of bacterial colonies produced after transformation of E. coli with DpnI-digested Hirt extracts of (A) HeLa and NSF cells, (B) WI38(SV40) and WI38 cells transfected for 72 hours with one of the following constructs (refer to Suppl. Table 3): pA3/4, p20mer1, p20mer1F, p20merC, pLB2, p20mer(LB2)1F, pLB2C1, p20mer(LB2C1)1F, and pBluescript. The average number of DpnI-resistant colonies per plate was corrected for the amount of DNA recovered and normalized to the number of colonies obtained with pA3/4 (positive control), which was taken as 100%. The number at the bottom of each bar denotes the average number of colonies obtained from 3 experiments. The error bars represent the average of 3 experiments performed in triplicate and 1 standard deviation.