Figure 7.

In vivo association of Brg-1 and Bmi-1 at the 20mer1, 20mer2, lamin B2, and c-myc chromosomal loci in transformed and normal cells. Histogram plots of the quantification by real-time PCR of immunoprecipitated DNA abundance (ng) at the 20mer1 (A), 20mer2 (B), lamin B2 (C), and c-myc (D) chromosomal loci in the 2 transformed {HeLa and WI38(SV40)} and 2 normal {NSF and WI38} cell lines. ChIP was performed with antibodies directed against Brg-1 (dark gray bars) and Bmi-1 (white bars); normal rabbit serum (NRS) (black bars) was used as a negative control. The primer sets of 20mer1 (A), 20mer2 (B), lamin B2 (C), and c-myc (D) are denoted in Suppl. Table 4. The error bars represent the average of at least 2 experiments performed in triplicate and 1 standard deviation. (E) Western blot analysis using anti-Brg-1 and anti-Bmi-1 antibodies to verify the immunoprecipitation of Brg-1 and Bmi-1 proteins in all cell lines used. Immunoprecipitation with NRS was used as a negative control.