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. 2012 Sep 26;2012:962652. doi: 10.1155/2012/962652

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Copyright © 2012 Zhenghui Wang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Live cell imaging of iNOS mRNA expression with the strand-displacement probes. Z-stack projection of confocal fluorescent images of RAW 264.7 cells and the quantitative analysis of fluorescence in selected regions of interests (ROIs) 24 h after transfection. The iNOS probe (0.4 μM FAM-iNOS-PNA annealed to 0.5 μM iNOS-DNA-DABCYL) or pLuc probe (0.4 μM FAM-pLuc-PNA annealed to 0.5 μM pLuc-DNA-DABCYL) was delivered with 9.7 μg/mL cSCK nanoparticle at an N/P ratio of 8 : 1. Green: FAM signal. Blue: Hoechst nuclear stain.